Journal: Journal of Inflammation Research

Article Title: Astragaloside IV Attenuates Angiotensin II-Induced Inflammatory Responses in Endothelial Cells: Involvement of Mitochondria

doi: 10.2147/JIR.S504427

Figure Lengend Snippet: Effect of astragaloside IV (AS-IV) on angiotensin II (Ang II)-induced monocyte adhesion to endothelial cells ( A and B ) and the activation of NF-κB ( C ) in endothelial cells. Human umbilical vein endothelial cells (HUVECs) were preincubated with or without AS-IV (3, 10, or 30 µM) 1 h before incubation with Ang II (1 µM) for 12 h or 30 min. Subsequently, HUVECs were coincubated with fluorescence-labeled human monocytes for 30 min or the NF-κB activity in HUVECs was assayed by the use of a nonradioactive ELISA-based assay kit. ( A ) Fluorescent images of monocyte adhesion to HUVECs were visualized with a fluorescence microscope at 485/535 nm. Magnification, × 200. HUVECs were incubated with (a) a vehicle (control), (b) Ang II alone, Ang II with AS-IV at the concentration of (c) 3, (d) 10, or (e) 30 µM before coincubation with fluorescence-labeled monocytes. ( B ) Fluorescence intensity of monocyte attachment to HUVECs was quantified using a spectrofluorometer. ( C ) The activities of NF-κB in HUVECs were assayed using a specific TransAM NF-κB p65 transcription factor assay kit. Results are expressed as means ± SE. * p < 0.05 compared with the control group; # p < 0.05 and ## p < 0.01 compared with HUVECs exposed to Ang II alone.

Article Snippet: The specific TransAM NF-κB p65 transcription factor assay kit and nuclear extraction kit were supplied by Active Motif (Carlsbad, CA, USA).

Techniques: Activation Assay, Incubation, Fluorescence, Labeling, Activity Assay, Enzyme-linked Immunosorbent Assay, Microscopy, Control, Concentration Assay, Transcription Factor Assay

Journal: Future Science OA

Article Title: Resveratrol alleviates depressive-like behavior via the activation of SIRT1/NF-κB signaling pathway in microglia

doi: 10.1080/20565623.2025.2463852

Figure Lengend Snippet: Resveratrol treatment suppressed the chronic unpredictable mild stress-induced changes of the protein levels of Sirt1, NF-κB p65, ac-p65, and Iκ-Bα in the mouse hippocampus via Western blot. *P < 0.05 vs. the control group; & P < 0.05 vs. the depression model group.

Article Snippet: NF-κB p65 DNA binding activity was detected using Trans AM NF-κB p65 Chemi Tran-scription Factor Assay Kit (Active Motif, Carlsbad, CA) according to the instructions of manufacturer.

Techniques: Western Blot, Control

Journal: Future Science OA

Article Title: Resveratrol alleviates depressive-like behavior via the activation of SIRT1/NF-κB signaling pathway in microglia

doi: 10.1080/20565623.2025.2463852

Figure Lengend Snippet: Resveratrol treatment suppressed the chronic unpredictable mild stress-induced changes of the protein levels of Sirt1, NF-κB p65, ac-p65, and Iκ-Bα in the mouse prefrontal cortex via Western blot. *P < 0.05 vs. the control group; & P < 0.05 vs. the depression model group.

Article Snippet: NF-κB p65 DNA binding activity was detected using Trans AM NF-κB p65 Chemi Tran-scription Factor Assay Kit (Active Motif, Carlsbad, CA) according to the instructions of manufacturer.

Techniques: Western Blot, Control

Journal: Future Science OA

Article Title: Resveratrol alleviates depressive-like behavior via the activation of SIRT1/NF-κB signaling pathway in microglia

doi: 10.1080/20565623.2025.2463852

Figure Lengend Snippet: Resveratrol treatment suppressed the LPS-induced changes of the protein levels of Sirt1, NF-κB p65, ac-p65, and Iκ-Bα in BV2 cells via Western blot. *P < 0.05 vs. the control group; **P < 0.01 vs. the control group; ***P < 0.001 vs. the control group.

Article Snippet: NF-κB p65 DNA binding activity was detected using Trans AM NF-κB p65 Chemi Tran-scription Factor Assay Kit (Active Motif, Carlsbad, CA) according to the instructions of manufacturer.

Techniques: Western Blot, Control

Journal: BMC Complementary Medicine and Therapies

Article Title: Combination effects of Pistachio hull and carfilzomib on NF-κB p65 , MDR1 , MRP1 , and Caspase3 gene expression in breast cancer cell line

doi: 10.1186/s12906-024-04716-7

Figure Lengend Snippet: Sequences of primers used in qPCR

Article Snippet: The concentration of the NF-κB p65 transcription factor was quantified according to the Zellbio kit protocol (Fig. ).

Techniques:

Journal: BMC Complementary Medicine and Therapies

Article Title: Combination effects of Pistachio hull and carfilzomib on NF-κB p65 , MDR1 , MRP1 , and Caspase3 gene expression in breast cancer cell line

doi: 10.1186/s12906-024-04716-7

Figure Lengend Snippet: The effects of pistachio hull extract (E), carfilzomib (CFZ), and their combination on NF-κB P65 gene expression levels in SK-BR3 and MCF10A cell lines. Statistical significance is indicated as follows: P < 0.05 * for all treatment groups compared to the control group (Tukey-HSD test); • P < 0.05 for tumor cell line compared to normal cell line (T-test); c P < 0.05 for comparisons among treatment groups (Tukey-HSD test); † P < 0.05 for comparisons between 24- and 48-hours within each category (T-test); ² P < 0.005; and ³ P < 0.001

Article Snippet: The concentration of the NF-κB p65 transcription factor was quantified according to the Zellbio kit protocol (Fig. ).

Techniques: Gene Expression, Control

Journal: BMC Complementary Medicine and Therapies

Article Title: Combination effects of Pistachio hull and carfilzomib on NF-κB p65 , MDR1 , MRP1 , and Caspase3 gene expression in breast cancer cell line

doi: 10.1186/s12906-024-04716-7

Figure Lengend Snippet: The effects of pistachio hull extract (E), carfilzomib (CFZ), and their combination on NF-κB p65 transcription factor levels in SK-BR3 and MCF10A cell lines. Statistical significance is indicated as follows: P < 0.05 * for all treatment groups compared to the control group (Tukey HSD test); • P < 0.05 for tumor cell line compared to normal cell line (T-test); c P < 0.05 for comparisons among treatment groups (Tukey HSD test); ² P < 0.005; and ³ P < 0.001

Article Snippet: The concentration of the NF-κB p65 transcription factor was quantified according to the Zellbio kit protocol (Fig. ).

Techniques: Control

Journal: JOR Spine

Article Title: Low‐Frequency Cyclic Stretch Upregulates the Expression of Nuclear Factor Erythroid 2‐Related Factor 2 in Human Nucleus Pulposus Cells to Inhibit the Resistin‐Induced Interleukin‐20 Expression

doi: 10.1002/jsp2.70040

Figure Lengend Snippet: Induction of NF‐kB‐p65 activity by resistin stimulation in NP cells. (A) IL‐20 mRNA expression levels were determined in NP cells pretreated with vehicle (DMSO) or SN50, or transfected with control siRNA (si‐CL) or si‐p65, and then stimulated with 50 ng/mL resistin for 2 h. RNA samples were isolated and subjected to real‐time polymerase chain reaction analysis. Data are presented as fold changes in fluorescent density from control NP cells (CL) normalized to 18S rRNA level. (B, C) The NF‐kB p65 activation was determined by a transcription factor (TF)‐enzyme‐linked immunosorbent assay. (B) NP cells were employed as control (CL) or stimulated with resistin for the durations indicated. (C) NP cells were pretreated individually with PD98059 (PD), SP600125 (SP), SB203580 (SB), or LY294002 (LY) for 1 h before being used as controls (CL) or stimulated with 50 ng/mL resistin for 1 h. NF‐κB p65 activity was then analyzed. All bar graphs represent folds of CL NP cells, mean ± standard error of the mean. * p < 0.05 versus CL. # p < 0.05 versus DMSO or si‐CL under resistin stimulation.

Article Snippet: NF‐κB p65 transcriptional activity was assessed by measuring its binding to specific DNA sequences using the NF‐κB (p65) Transcription Factor Assay Kit (Cayman Chemical, Ann Arbor, MI, USA).

Techniques: Activity Assay, Expressing, Transfection, Control, Isolation, Real-time Polymerase Chain Reaction, Activation Assay, Enzyme-linked Immunosorbent Assay

Journal: JOR Spine

Article Title: Low‐Frequency Cyclic Stretch Upregulates the Expression of Nuclear Factor Erythroid 2‐Related Factor 2 in Human Nucleus Pulposus Cells to Inhibit the Resistin‐Induced Interleukin‐20 Expression

doi: 10.1002/jsp2.70040

Figure Lengend Snippet: Blockade of TLR4 activity inhibited resistin‐induced IL‐20 expression. NP cells were kept as controls (CL), or pretreated with isotype‐matched IgG (Ab‐IgG) and specific TLR4 neutralizing antibody (Ab‐TLR4), or transfected with the control siRNA (si‐CL) and si‐TLR4, and subsequently stimulated with resistin for 2 h (A) and 1 h (B). (A) IL‐20 mRNA levels were determined through real‐time PCR in NP cells and normalized to 18S rRNA. (B) The activation of NF‐kB‐p65 in NP cells after resistin stimulation was analyzed using transcription factor (TF) ELISA. All bar graphs represent folds of control NP cells (CL), mean ± standard error of the mean. * p < 0.05 versus CL NP cells. # p < 0.05 versus IgG‐pretreated or si‐CL‐transfected NP cells under resistin stimulation.

Article Snippet: NF‐κB p65 transcriptional activity was assessed by measuring its binding to specific DNA sequences using the NF‐κB (p65) Transcription Factor Assay Kit (Cayman Chemical, Ann Arbor, MI, USA).

Techniques: Activity Assay, Expressing, Transfection, Control, Real-time Polymerase Chain Reaction, Activation Assay, Enzyme-linked Immunosorbent Assay

Journal: JOR Spine

Article Title: Low‐Frequency Cyclic Stretch Upregulates the Expression of Nuclear Factor Erythroid 2‐Related Factor 2 in Human Nucleus Pulposus Cells to Inhibit the Resistin‐Induced Interleukin‐20 Expression

doi: 10.1002/jsp2.70040

Figure Lengend Snippet: Pre‐exposure of NP cells to 5% cyclic stretch with 0.1 Hz for 30 min inhibited resistin‐induced IL‐20 expression. Static NP cells were stimulated with resistin without prestretching (static). NP cells were kept as controls (CL) or pre‐exposed to cyclic stretch at 5% with 0.1 Hz for the indicated durations followed by resistin stimulation. (A) The mRNA levels of IL‐20 in NP cells were determined through real‐time polymerase chain reaction and normalized to 18S rRNA. * p < 0.05 versus CL NP cells. ** p < 0.05 versus static NP cells with resistin stimulation. # p < 0.05 versus resistin‐treated NP cells with cyclic stretch at 5% with 0.1 Hz for 10' and 1 h. (B) The phosphorylation of p38 MAPK and Akt was determined by Western blotting. (C) NF‐kB‐p65 activation in NP cells after 1 h resistin stimulation was analyzed by TF‐ELISA. All bar graphs represent folds of control NP cells (CL), mean ± standard error of the mean. * p < 0.05 versus CL NP cells. # p < 0.05 versus static NP cells with resistin stimulation.

Article Snippet: NF‐κB p65 transcriptional activity was assessed by measuring its binding to specific DNA sequences using the NF‐κB (p65) Transcription Factor Assay Kit (Cayman Chemical, Ann Arbor, MI, USA).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Phospho-proteomics, Western Blot, Activation Assay, Enzyme-linked Immunosorbent Assay, Control

Journal: JOR Spine

Article Title: Low‐Frequency Cyclic Stretch Upregulates the Expression of Nuclear Factor Erythroid 2‐Related Factor 2 in Human Nucleus Pulposus Cells to Inhibit the Resistin‐Induced Interleukin‐20 Expression

doi: 10.1002/jsp2.70040

Figure Lengend Snippet: Upregulation of NRF2 inhibited resistin‐induced IL‐20 expression in NP cells. (A) NP cells were used as static control (static) or exposed to 5% with 0.1 Hz cyclic stretch for 30 min or 2 h. The expression of NRF2 in the nucleus was determined by Western blotting. (B–D) NP cells were used as static control (static), or pre‐exposed to 5% with 0.1 Hz for 30 min, and then treated with resistin (50 ng/mL) for 2 h (B), 30 min (C), and 1 h (D). Prior to cyclic stretch exposure, NP cells were transfected with the control siRNA (si‐CL) or si‐NRF2. (B) The levels of IL‐20 mRNA in NP cells were determined through real‐time polymerase chain reaction and normalized to 18S rRNA. (C) The phosphorylation of p38 MAPK, and Akt was determined by Western blotting. (D) NF‐kB‐p65 activation in NP cells was analyzed by TF‐ELISA. All bar graphs represent the percentage of static NP cells (static), mean ± standard error of the mean. * p < 0.05 versus static NP cells.

Article Snippet: NF‐κB p65 transcriptional activity was assessed by measuring its binding to specific DNA sequences using the NF‐κB (p65) Transcription Factor Assay Kit (Cayman Chemical, Ann Arbor, MI, USA).

Techniques: Expressing, Control, Western Blot, Transfection, Real-time Polymerase Chain Reaction, Phospho-proteomics, Activation Assay, Enzyme-linked Immunosorbent Assay